{"Equipment":"<ul>\r\n\t<li>\r\n\t\tCorkplate or wax board</li>\r\n\t<li>\r\n\t\tpins</li>\r\n\t<li>\r\n\t\tjar containing tap water to rinse tools</li>\r\n\t<li>\r\n\t\tlabelled container with fixative</li>\r\n\t<li>\r\n\t\tfine scissors</li>\r\n\t<li>\r\n\t\tbigger scissors</li>\r\n\t<li>\r\n\t\tforceps</li>\r\n\t<li>\r\n\t\tsmall spatula</li>\r\n\t<li>\r\n\t\tscalpel</li>\r\n\t<li>\r\n\t\tlaboratory balance</li>\r\n</ul>\r\n","Procedure":"<ol>\n\t<li>\n\t\tSacrifice the mouse</li>\n\t<li>\n\t\tWeigh and record total body weight (if not done separately)</li>\n\t<li>\n\t\tPlace mouse on its back and pin the mouse onto board with extended extremities (inner side of hands and foot)</li>\n\t<li>\n\t\tWipe or spray the mouse with 70% ethanol to control hair and dander</li>\n\t<li>\n\t\tProceed with a complete necropsy and tissue collection according to Center-specific technical SOP but be sure to collect the IMPC &#39;Tissue Collection List&#39; (below) and remove the heart and record its weight according to the IMPC Heart Weight SOP. Inidividual Centers may collect any additional tissue according to Centre-specific technical SOPs.</li>\n\t<li>\n\t\tCapture any images of abnormal findings and save images in Centre-specific database</li>\n\t<li>\n\t\tPlace all tissue collected in a labelled jar containing a sufficient volume of fixative (minimum of 10:1 fixative to tissue)</li>\n\t<li>\n\t\tRecord all abnormal findings in a Center-specific database using the standardized IMPC Gross Pathology ontology:\n\t\t<ol type=\"i\">\n\t\t\t<li>\n\t\t\t\tMA ontology terms for location/topography</li>\n\t\t\t<li>\n\t\t\t\tMP ontology terms for presence/absence/large/small/abnormal</li>\n\t\t\t<li>\n\t\t\t\tPATO ontology terms for descriptors (size, shape, colour, consistency, distribution of lesions)</li>\n\t\t</ol>\n\t</li>\n</ol>\n","Purpose":"<p>\r\n\tTo perform a complete necropsy to detect and record abnormal external findings and macroscopic alterations in internal and external organs, record body and heart weights (see IMPC Heart Weight SOP), and collect a standardized list of tissues for fixation with or without further processing (see non-mandatory IMPC Tissue Embedding &amp; Block Banking SOP).</p>\r\n","Experimental Design":"<div class=\"expandable\">\n\t<ul>\n\t\t<li>\n\t\t\t<strong>Minimum number of animals :</strong>&nbsp; 2M + 2F</li>\n\t\t<li>\n\t\t\t<strong>Age at test: </strong>Week 60 </li></ul>\n</div>\n<p>\n\t&nbsp;</p>\n","Notes":"<p>\n\tBanking of the fixed tissue for long-term storage is according to Centre-specific SOPs (including storage fixative). Options for long-term storage of the fixed tissue include:</p>\n<ul>\n\t<li>\n\t\tStore in labelled jar in wet fixative for future use or requests</li>\n\t<li>\n\t\tTrim, process, and embed fixed tissue in paraffin blocks (store or discard tissue not trimmed in)</li>\n\t<li>\n\t\tTrim, process, and embed fixed tissue in paraffin blocks followed by microtomy and staining of sections on glass slides (store or discard tissue not trimmed in)</li>\n</ul>\n<p>\n\t<br />\n\t<strong>Data QC</strong><br />\n\t<br />\n\tImages of significant gross findings will be captured as static image files (.tiff or .jpg) and made available to the DCC.&nbsp; Images will be not be annotated but linked by metadata to the mouse line.</p>\n"}