{"Equipment":"<ol>\n\t<li>\n\t\tScale/balance</li>\n\t<li>\n\t\tFlow chambers</li>\n\t<li>\n\t\tPlethysmographs</li>\n\t<li>\n\t\tNebulizers</li>\n\t<li>\n\t\tComputer connected to flow chamber</li>\n\t<li>\n\t\tChallenge reagents</li>\n\t<li>\n\t\tPersonal safety equipment (masks, gloves, etc.)</li>\n</ol>\n","Procedure":"<p>\n\tSensitization:</p>\n<ul>\n\t<li>\n\t\tDay 0: administrate OVA (20 &micro;g) + Alum (2mg ) ip</li>\n\t<li>\n\t\tDay 7: administrate OVA (20 &micro;g) + Alum (2mg ) ip</li>\n\t<li>\n\t\tDay 14: administrate aerosol 2.5% OVA (40min/day)</li>\n\t<li>\n\t\tDay 15/16: administrate aerosol 2.5% OVA (40min/day)</li>\n\t<li>\n\t\tDay 17/18: administrate aerosol 2.5% OVA (40min/day)</li>\n\t<li>\n\t\tDay 20: administrate aerosol 2.5% OVA (40min/day)</li>\n</ul>\n<div>\n\t<div>\n\t\tOn the day of the Whole Body Plethysmography procedure:</div>\n\t<ol>\n\t\t<li>\n\t\t\tTransfer the cohort to be tested to the test room.</li>\n\t\t<li>\n\t\t\tWeigh each mouse in the cohort and record weight.</li>\n\t\t<li>\n\t\t\tTurn on amplifier, nebulizer&nbsp;and computer,&nbsp;and calibrate the equipment.</li>\n\t\t<li>\n\t\t\tPlace each mouse in the cohort in an individual plethysmograph chamber and acclimatize for 30 minutes.</li>\n\t\t<li>\n\t\t\tMethacholine (MCh) challenge:\n\t\t\t<ul style=\"\">\n\t\t\t\t<li>\n\t\t\t\t\tMeasure and record baseline data for 5 minutes</li>\n\t\t\t\t<li>\n\t\t\t\t\tNebulize with PBS for 2 minutes</li>\n\t\t\t\t<li>\n\t\t\t\t\tMeasure and record response to PBS for 5 minutes</li>\n\t\t\t\t<li>\n\t\t\t\t\tNebulize with 12.5mg/ml MCh for 2 minutes</li>\n\t\t\t\t<li>\n\t\t\t\t\tMeasure and record response to 12.5mg/ml for 5 minutes</li>\n\t\t\t\t<li>\n\t\t\t\t\tNebulize with 25mg/ml MCh for 2 minutes</li>\n\t\t\t\t<li>\n\t\t\t\t\tMeasure and record response to 25mg/ml for 5 minutes</li>\n\t\t\t\t<li>\n\t\t\t\t\tNebulize with 50mg/ml MCh for 2 minutes</li>\n\t\t\t\t<li>\n\t\t\t\t\tMeasure and record response to 50mg/ml MCh for 5 minutes</li>\n\t\t\t</ul>\n\t\t</li>\n\t\t<li>\n\t\t\t<div>\n\t\t\t\tRemove each mouse from its chamber and place back in the home cage.</div>\n\t\t</li>\n\t\t<li>\n\t\t\t<div>\n\t\t\t\tSacrifice the mouse and collect samples:</div>\n\t\t\t<ul style=\"\">\n\t\t\t\t<li>\n\t\t\t\t\t<div>\n\t\t\t\t\t\tWhole blood by cardiac punture&nbsp;to prepare for serum analysis of IgE and IgG</div>\n\t\t\t\t</li>\n\t\t\t\t<li>\n\t\t\t\t\t<div>\n\t\t\t\t\t\tBronchoalveolar lavage (BAL) fluid into 1.5ml eppendorf tube&nbsp;for analysis of inflammatory cells and cytokines</div>\n\t\t\t\t</li>\n\t\t\t\t<li>\n\t\t\t\t\t<div>\n\t\t\t\t\t\tWhole lung tissue immersion fixation in 10% NBF for histopathology</div>\n\t\t\t\t</li>\n\t\t\t</ul>\n\t\t</li>\n\t</ol>\n</div>\n<p>\n\t&nbsp;</p>\n","Purpose":"<p>\n\tThe purpose of this procedure is to record the respiratory function of mice after sensitization with an inert allergen (ovalbumin; OVA) in response to challenge with a non-allergen airway agonist (Methacholine; MCh) using unrestrained whole-body plethysmography and assess the ability of the immune system to mount an acquired Type I allergic hypersensitivity immune response (allergic airway disease).</p>\n","Experimental Design":"<div class=\"expandable\">\n\t<ul>\n\t\t<li>\n\t\t\t<strong>Minimum number of animals :</strong>&nbsp; 6M + 6F</li>\n\t\t<li>\n\t\t\t<strong>Age at test: </strong>Week 11 </li>\n\t\t<li>\n\t\t\t<strong>Sex:</strong> We would expect the results of this test to show sexual dimorphism</li>\n\t</ul>\n</div>\n<p>\n\t&nbsp;</p>\n"}