{"Equipment":"
    \n\t
  1. \n\t\tFlow chambers
    2. \n\t
  2. \n\t\tPlethysmographs
    3. \n\t
  3. \n\t\tComputer connected to flow chamber
    4. \n\t
  4. \n\t\tChallenge reagents
    5. \n\t
  5. \n\t\tPersonal safety equipment (masks, gloves, etc.)
    6. \n
\n","Procedure":"
    \n\t
  1. \n\t\tBefore starting, calibrate the plethysmographic chambers
    2. \n\t
  2. \n\t\tFreshly prepare a 200 µg/mL LPS (O55:B5 serotype) solution in sterile saline (NaCl 0.9 %).
    3. \n\t
  3. \n\t\tInject 50 mg/kg ketamine and 3.3 mg/kg xylazine to mice in 100 µL volume by intraperitoneal injection
    4. \n\t
  4. \n\t\tAdministrate 25 µL of LPS solution (5 µg / mice)
    5. \n\t
  5. \n\t\tMouse are next placed in decubitus on a heated plate during 10-15 min 
    6. \n\t
  6. \n\t\tPlace mice in the chambers
    7. \n\t
  7. \n\t\tStart the experimental recording phase (6 hours)
    8. \n\t
  8. \n\t\tRemove mice from chambers and place back in the home cages.
    9. \n
\n","Purpose":"

\n\tThe purpose of this procedure is to record the respiratory function of mice, with a LPS challenge, by means of Unrestrained Whole-Body Plethysmography, using a lipopolysaccharides (LPS) challenge.

\n
\n\tDescription:  Spontaneous bronchospasm after LPS challenge
\n

\n\t 

\n","Experimental Design":"
\n\t\n
\n

\n\t 

\n"}