{"Equipment":"<ol>\r\n\t<li>\r\n\t\tELISA plate reader / MSD Sector Imager</li>\r\n\t<li>\r\n\t\tVortex</li>\r\n\t<li>\r\n\t\tRefrigerated centrifuge</li>\r\n\t<li>\r\n\t\tEppendorf tubes</li>\r\n\t<li>\r\n\t\tCalibrated Pipettes</li>\r\n</ol>\r\n","Procedure":"<ol>\r\n\t<li>\r\n\t\tBlood is collected by the relevant blood collection procedure (see IMPC protocol &quot;Blood collection by retro-orbital puncture&quot;). Blood is collected in lithium heparin tubes and the samples are kept on ice for a maximum of 2 hours prior to isolation of the plasma.</li>\r\n\t<li value=\"2\">\r\n\t\tBlood samples are centrifuged at 5,000 x g for 10 minutes at 8&ordm;C and the plasma removed and aliquoted for analysis or for freezing (-70&ordm;C).</li>\r\n\t<li value=\"3\">\r\n\t\tPlasma samples are subsequently defrosted and the required amount of sample is used to perform the analysis (e.g. by ELISA or MSD).</li>\r\n</ol>\r\n","Purpose":"<p>\r\n\tThe insulin concentration in the blood is an important indicator of diabetes.</p>\r\n<p>\r\n\tOntological description: abnormal circulating insulin level [MP:0001560]; increased circulating insulin level [MP:0002079]; decreased circulating insulin level [MP:0002727].</p>\r\n","Experimental Design":"<p>\n\tAge of animal: Males at 33,57 and 72 weeks.</p>\n<p>\n\tSexual dimorphism: Yes.</p>\n","Notes":"<p>\r\n\tBlood collection for Insulin Blood Level is performed as a non-fasting, terminal procedure.</p>\r\n<p>\r\n\t<strong>Data QC</strong></p>\r\n<ol>\r\n\t<li>\r\n\t\tPlasma samples must be free of Fibrin clots in order to be analysed.</li>\r\n\t<li>\r\n\t\tBadly hemolysed samples should not be included in the analysis.</li>\r\n</ol>\r\n<p>\r\n\t<strong>Example Metadata</strong></p>\r\n<table border=\"1\" cellpadding=\"1\" cellspacing=\"0\" style=\"width:700px\">\r\n<thead>\r\n\t\t<tr>\r\n\t\t\t<th scope=\"col\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tMetadata</p>\r\n\t\t\t</th>\r\n\t\t\t<th scope=\"col\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tExample</p>\r\n\t\t\t</th>\r\n\t\t\t<th scope=\"col\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tRequired for data upload</p>\r\n\t\t\t</th>\r\n\t\t\t<th scope=\"col\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tRequired for data analysis</p>\r\n\t\t\t</th>\r\n\t\t</tr>\r\n</thead>\r\n<tbody>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tType of kit</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tThe kit used for analysing the blood samples. E.g. Mouse Insulin kit</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tNO</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tKit manufacturer</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tManufacturer of the kit. E.g. MORINAGA (Yokohama, Japan)</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tNO</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tKit lot number</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\t&nbsp;</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tNO</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tEquipment ID</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tID of the machine used when more than 1 is used having same model and manufacturer. E.g. machine 1, machine 2, machine Minnie, machine Mickey Mouse, etc.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tNO</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tEquipment manufacturer</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tManufacturer of the equipment. E.g. Thermo scientific.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;height:27px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tEquipment model</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;height:27px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tModel of the equipment. E.g. Multiskan JX.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;height:27px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;height:27px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;height:43px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tBlood collection tubes</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;height:43px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tThe tubes used for blood collection. E.g. Sarstedt Li-Heparin gel tubes or Kabe Labortechnik Lithium heparin coated tubes.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;height:43px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tNO</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;height:43px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;height:43px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tAnesthesia used for blood collection</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;height:43px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tThe anesthetic used during blood collection. E. g. Isofluorane.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;height:43px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;height:43px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;height:43px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tMethod of blood collection</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;height:43px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tConcise description of the method used for blood collection. E.g. retro-orbital puncture.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;height:43px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;height:43px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tAnticoagulant</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tAnticoagulant used for blood collection. E.g. Li-Heparin.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tDate and time of blood collection</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tTime of day for collection is in the morning, starting no earlier than 07:30. E.g. Year, month, day, time.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tDate of measurement</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tThe day of blood analysis. Year, month, day.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tSample status</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tIndicate if the sample were frozen (analysis on the same day of collection not possible) or fresh (analysis on the same day of collection). E.g. Fresh/Frozen.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tSamples kept on ice between collection and analysis</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYes/No.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tPlasma dilution</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tDilution is highly discouraged but if necessary indicate here. E.g. &quot;No dilution&quot; or 1:2. Note that results submitted to DCC are assumed to be already corrected for any dilutions made.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tReplicates</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tPlease specify whether samples were measured once, in duplicate or in triplicate. E.g. 1 or 2 or 3.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tNO</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tID of blood collection SOP</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tID of the protocol followed for blood collection. Can be a centre specific protocol. E.g. ESLIM_024_001.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tHemolysis status</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tThe gauged degree of hemolysis. E.g. slight/moderate/marked.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tNO</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tBlood collection experimenter ID</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tAn ID of any format to be used coherently both inside the same procedure and for all procedures indicating the experimenter who collected the blood. E.g. Harw_001, or 1/2/3.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tNO</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tBlood analysis experimenter ID</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tAn ID of any format to be used coherently both inside the same procedure and for all procedures indicating the experimenter who analyzed the blood. E.g. Harw_001, or 1/2/3.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tYES</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;height:33px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tNO</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n\t\t<tr>\r\n\t\t\t<td style=\"width:145px;height:37px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tDate equipment last calibrated</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:217px;height:37px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tMost recent date in which the equipment (or any part of) used in the procedure was subject to a calibration event.</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:107px;height:37px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tNO</p>\r\n\t\t\t</td>\r\n\t\t\t<td style=\"width:139px;height:37px;\">\r\n\t\t\t\t<p>\r\n\t\t\t\t\tNO</p>\r\n\t\t\t</td>\r\n\t\t</tr>\r\n</tbody>\r\n</table>"}