{"Procedure":"<ol><li>Habituation</li><ol type = \"a\"><li>Place mice in the testing chambers on the elevated wire grid.</li><li>Leave each mouse for an hour to acclimatise.</li><li>The left hind paw is tested first using the following method.</li><ol type=\"i\"><li>The 0.6g von Frey filament should be used as the starting filament.</li><li>Apply the filament to the plantar surface of the hind paw. The filament should be applied with enough force to cause the filament to bend, and remain in contact for a total of 1-2 seconds.</li><li>The size of the next filament to be applied will depend on the response to the previous filament.</li><ul><li>If no withdrawal response was observed then the next highest filament is tested.</li><li>If there was a withdrawal response then the next lowest filament is tested.</li></ul><li>Continue applying filaments until the paw has been tested 5 times, with at least 2 minutes between each stimulus presentation.</li></ol><li>Repeat the testing process with the right hind paw.</li></ol><li>Test 1</li><ol type = \"a\"><li>24 hours after habituation, re-test the mouse with the von Frey filaments using the same procedure as described for the habituation period.</li></ol><li>Test 2</li><ol type = \"a\"><li>48 hours after habituation, re-test the mouse with the von Frey filaments using the same procedure as described for the habituation period.</li></ol></ol>","Purpose":"<p>To assess mechanical sensitivity using calibrated von Frey filaments.</p>","Experimental Design":"<p>Minimum number of mutant animals: 7 males + 7 females </p>\r\n<p>Age at test: 10 weeks</p>\r\n<p>Sexual dimorphism:</p>","Notes":"<p><b>This procedure is a pilot study from the Pain Phenotyping Pilot</b></p>"}