{"Procedure":"<ol> <li>Baseline measurement</li><ol type = \"a\"><li>Set up the testing room and equipment. Check the condition of the von Frey filaments.</li><li>Place mice into the testing arenas and leave them to habituate for the specified length of time.</li><li>Once habituated, test the mouse with the starting filament. When the mouse has all four feet on the platform, position the starting filament below the mid-plantar surface of the foot.</li><li>Press the filament against the foot until the filament bends. Hold the filament in position for 3 seconds, or until the mouse moves its foot.</li><li>Mark the response as &#39;0&#39; if the mouse does not react, or &#39;X&#39; if it does react to the filament.</li><li>Move on to testing the next mouse with the starting filament.</li><li>Once all mice have been tested with the starting filament, test them with the next filament.</li><ul><li>If the mice did respond to the previous filament, they should be tested with the filament of the next smallest size. Once tested, record the response.</li><li>If the mice did not respond to the previous filament, they should be tested with the filament of the next largest size. Once tested, record the response.</li></ul><li>Continue testing the mice using the up-down pattern until the stated number of trials have been completed.</li></ol><li>Challenge</li><ol type = \"a\"><li>The challenge should be administered 24 hours after the baseline measurement took place.</li><li>Prepare the challenge injection.</li><li>Anaesthetise the mouse.</li><li>Administer the challenge to the plantar surface of the right hind paw.</li><li>Monitor the condition of the mouse as it recovers from the anaesthetic.</li></ol><li>Test 1</li><ol type = \"a\"><li>24 hours after the challenge injection, re-test the mouse with the von Frey filaments using the same procedure as described for the baseline measurement.</li></ol><li>Test 2</li><ol type = \"a\"><li>48 hours after the challenge injection, re-test the mouse with the von Frey filaments using the same procedure as described for the baseline measurement.</li></ol></ol>","Purpose":"<p>To investigate mechanical sensitivity in wild type and genetically altered mice.</p>","Experimental Design":"<p>Minimum number of mutant animals: 7 males + 7 females </p>  <p>Age at test: 17 weeks</p>  <p>Sexual dimorphism:</p>","Notes":"<p><b>This procedure is a pilot study from the Pain Phenotyping Pilot</b></p>"}