{"Procedure":"<ol><li>Acclimatise the mice to the arena for 30 seconds on the day before testing.</li><li>On the day of the test, switch on the hot plate and bring up the temperature to 52&#176; Celsius. Two people should be present for the test.</li><li>Set up the video recording camera to record a 360&#176; view of the hot plate, using mirrors to reflect the images.</li><li>Place the mouse on the hot plate, depressing the foot pedal simultaneously.</li><li>Both experimenters observe the mouse from 2 different angles for any reaction to the stimulus.</li><ul><li>If a reaction is observed then the foot pedal is depressed again and the mouse is removed from the hot plate.</li><li>The mouse is removed after 30 seconds, even if no reaction has been observed.</li></ul><li>The latency to response and the type of response are manually recorded using the video output.</li></ol>","Purpose":"<p>To assess thermal sensitivity.</p>","Notes":"<p><b>This procedure is a pilot study from the Pain Phenotyping Pilot</b></p>","Experimental Design":"<p>Minimum number of mutant animals: 7 males + 7 females </p><p>Age at test: 9 weeks</p><p>Sexual dimorphism:</p>"}