{"Procedure":"<ol><li>Baseline measurement</li><ol type = \"a\"><li>Move the mice to the testing chambers and leave to acclimate for the specified length of time.</li><li>Mice should be tested using the simplified up-down method (SUDO) of Bonin et al. (2014).</li><li>When the mouse is still, apply the filament to the centre of the right hindpaw. Press the filament against the paw for 3 seconds. </li><li>Mark the response &#39;0&#39; if the mouse does not react, or &#39;X&#39; if it does react to the filament.</li><li>Leave the mouse for a minimum of 2 minutes before presentation of the next filament.</li><li>The next filament to be tested will depend on the response to the previous filament.</li><ul><li> If the mice did respond to the previous filament, they should be tested with the filament of the next smallest size. Once tested, record the response.</li><li>If the mice did not respond to the previous filament, they should be tested with the filament of the next largest size. Once tested, record the response.</li></ul><li>Continue testing the mice until 5 trials have been completed. </li><li>The procedure is then repeated for a second run.</li></ol><li>Challenge</li><ol type = \"a\"><li>The challenge is administered after the baseline measurement has been completed.</li></ol><li>Test 1</li><ol type = \"a\"><li>22 hours after the challenge injection, re-test the mouse with the von Frey filaments using the same procedure as described for the baseline measurement.</li></ol><li>Test 2</li><ol type = \"a\"><li>142 hours after the challenge injection, re-test the mouse with the von Frey filaments using the same procedure as described for the baseline measurement.</li></ol></ol>","Purpose":"<p>To detect mechanical/tactile sensitivity in the mouse paw</p>","Experimental Design":"<p>Minimum number of mutant animals: 7 males + 7 females </p><p>Age at test: 16 weeks</p><p>Sexual dimorphism:</p>","Notes":"<p><b>This procedure is a pilot study from the Pain Phenotyping Pilot</b></p>\r\n<p>A simplified up-down method (SUDO) for measuring mechanical nociception in rodents using von Frey filaments. Bonin RP, Bories C, De Koninck Y. Mol Pain. 2014 Apr 16;10:26. doi: 10.1186/1744-8069-10-26.PMID:24739328</p>"}