{"Procedure":"<ol><li>Baseline measurement</li><ol type = \"a\"><li>Transfer mouse to the testing arena and allow to acclimate to the test environment.</li><li>Ensure that the intensity and cut-off timer of the radiant heat source are set correctly.</li><li>Position the light source below the platform and directed at the correct part of the paw.</li><li>Turn the light source on and record the latency to paw withdrawal. The stimulus is terminated once a response is registered, or if the maximum exposure time is reached before the animal responds.</li><li>Repeat the test so that each mouse is tested three times with the same paw.</li></ol><li>Challenge</li><ol type = \"a\"><li>The challenge is administered after the baseline measurement has been completed.</li><li>Anaesthetise the mouse and administer the challenge injection.</li></ol><li>Test 1</li><ol type = \"a\"><li>After the specified length of time since the challenge injection, the mouse is re-tested using the same procedure as described for the baseline measurement.</li></ol><li>Test 2</li><ol type = \"a\"><li>After the specified length of time since the challenge injection, the mouse is re-tested again using the same procedure as described for the baseline measurement.</li></ol></ol>","Purpose":"<p>To assess thermal hyperalgesia in the mouse paw.</p>","Experimental Design":"<p>Minimum number of mutant animals: 7 males + 7 females </p>  <p>Age at test: 16-17 weeks</p>  <p>Sexual dimorphism:</p>","Notes":"<p><b>This procedure is a pilot study from the Pain Phenotyping Pilot</b></p>"}