{"Procedure":"<ol>\n\t<li>\n\t\t<strong>Set up timed mating with heterozygous animals. Dissect and collect &gt;=3 homozygote embryos. </strong></li>\n\t<li>\n\t\t<strong>Process the embryos using the centre&rsquo;s staining and embedding protocol . Centre-specific methods&nbsp; are recorded within a document</strong></li>\n\t<li>\n\t\t<strong>Scan and generate reconstructions of embryos.</strong></li>\n</ol>\n","Purpose":"<p>\tTo assess morphological defects in E9.5 embryos from lethal strains with MicroCT.</p>","Experimental Design":"<ul>\t<li>\t\tSet up timed matings with heterozygous mice</li>\t<li>\t\tDay 0 is defined as the midpoint of the prior dark cycle following the identification of a copulation plug.</li>\t<li>\t\tCollect embryos at E9.5 with 3 minimum homozygous and 1 littermate control</li>\t<li>\t\tEnsure embryos are bleed as much as possible.</li>\t<li>\t\tReconstruct the scans according to manufacturer&#39;s instructions.</li></ul>","Notes":"<p>\n\tEmbryos may be processed for Histopathology.</p>\n"}