{"Data QC":"<p>\n\tLocal QC data to assure all data fields have recorded data.&nbsp; In the case all data fields have a value of 0 that particular mouse data will be removed.</p>\n<p>\n\t1.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Verify the total time of each run</p>\n","Procedure":"<p>1. Acclimatise mice for at least 1 hour before test.</p> <p>2. Clean the arena with disinfectant and ensure arena is dry.</p> <p>3. Light intensity levels are set at 200 lux at the centre of light compartment and at 5 lux at the dark one. Light adjustment is performed at the start of each test day.</p> <p>4. The method of recording is set up with each arena defined. The dark arena constitute approximately 40% of entire chamber.</p> <p>5. One mouse is placed in the dark compartment and recording is started.</p> <p>6. The test lasts 5 minutes.</p> <p>7. At the end of the trial, the mice are removed from the arena and returned to their home cages.</p> <p>8. Number of transition and time spent in each compartment are calculated automatically by software.</p>","Purpose":"<p>\tTo monitor the anxiety-related behaviour of mice in a two-chambered light-dark arena over a defined period of time in order to assess the anxiety phenotype.","Experimental Design":"<div class=\"expandable\">\n\t<ul>\n\t\t<li>\n\t\t\t<strong>Minimum number of animals :</strong>&nbsp; 7M + 7F</li>\n\t\t<li>\n\t\t\t<strong>Age at test: </strong>Week 10 </li>\n\t\t<li>\n\t\t\t<strong>Sex:</strong> We would expect the results of this test to show sexual dimorphism</li>\n\t</ul>\n</div>\n<p>\n\t&nbsp;</p>\n","Notes":"<p>\n\tLocal data processing may be implemented to calculate derived measures from this data.</p>\n"}