{"Procedure":"
    \n\t
  1. \n\t\tExamine the anterior of both eyes (e.g. with slit lamp) and record any abnormalities
    2. \n\t
  2. \n\t\tTest the iris/pupil light response
    3. \n\t
  3. \n\t\tImage abnormal eyes as a minimum or all eyes if capacity permits
    4. \n\t
  4. \n\t\tDilate both eyes
    5. \n\t
  5. \n\t\tExamine the anterior and posterior of both dilated eyes (e.g. with slit lamp and ophthalmoscope) and record any abnormalities
    6. \n\t
  6. \n\t\tImage abnormal eyes as a minimum or all eyes if capacity permits
    7. \n
\n

\n\tOCT:

\n
    \n\t
  1. \n\t\tTurn on the OCT and start the database
    2. \n\t
  2. \n\t\tAnaesthetize mouse
    3. \n\t
  3. \n\t\tPrepare mouse eyes with drops and place contact lens (focal length 10 mm) on the right eye
    4. \n\t
  4. \n\t\tEnter mouse data in the “Create new patient file” area and switch to the “Acquisition” window
    5. \n\t
  5. \n\t\tMove the OCT camera to the right position and activate measurement modus
    6. \n\t
  6. \n\t\tPlace mouse collaterally to the OCT camera on the right side of a platform that is fixed in front of the OCT lens
    7. \n\t
  7. \n\t\tSearch the contact lens in the live picture of the fundus image field and place the pupil of the mouse eye in the centre of the window
    8. \n\t
  8. \n\t\tMove the OCT camera such that OCT lens and contact lens touch each other
    9. \n\t
  9. \n\t\tFocus the fundus picture by slightly moving up/down or forward/backward
    10. \n\t
  10. \n\t\tSave fundus images
    11. \n\t
  11. \n\t\tSet the "Ref.Arm“ ruler such that the section of the retina is placed in the centre of the blue rectangle
    12. \n\t
  12. \n\t\tSet the mode of measurement on "vertical, horizontal line“
    13. \n\t
  13. \n\t\tMove the blue horizontal line in the fundus image field to the optic nerve level
    14. \n\t
  14. \n\t\tSave images of retinal sections
    15. \n\t
  15. \n\t\tMove the OCT camera to the left position
    16. \n\t
  16. \n\t\tRepeat measurement procedure for the left eye
    17. \n
\n

\n\tScheimpflug Imaging:

\n
    \n\t
  1. \n\t\tTurn on the Pentacam and start the patient data management
    2. \n\t
  2. \n\t\tApply one drop 0.5% Atropine to each mouse eye for pupil dilation
    3. \n\t
  3. \n\t\tEnter mouse data in the “Patient” group box and switch to the Scan menu
    4. \n\t
  4. \n\t\tActivate the “1 Picture” modus in the “Image Options” area
    5. \n\t
  5. \n\t\tMove Pentacam to the right position
    6. \n\t
  6. \n\t\tHold the mouse on a platform such that the vertical LED 475 nm light slit is orientated in the center of the right eye ball
    7. \n\t
  7. \n\t\tGuarantee optimal focus by using the fine adjustment software tool in the adjustment window
    8. \n\t
  8. \n\t\tStart imaging manually by pressing the “Start Scan” button
    9. \n\t
  9. \n\t\tScheimpflug images are saved automatically
    10. \n\t
  10. \n\t\tMove Pentacam to the left position
    11. \n\t
  11. \n\t\tRepeat measurement procedure for the left eye
    12. \n
\n","Purpose":"

\r\n\tTo detect abnormalities in eye morphology.

\r\n","Experimental Design":"
\n\t\n
\n

\n\t 

\n","Notes":"\r\n\r\n\r\n
\r\n

Data QC

\r\n

Image QC is typically performed during data collection to ensure high quality images are captured whilst eyes are dilated etc.

"}