{"Procedure":"1. Acclimatise mice in the testing room for at least 30 minutes before testing.<br />2. Anaesthetize mouse and administer formalin with a subcutaneous injection into the plantar surface of the chosen paw.<br />3. Place the mouse into the testing arena and start the recording. Run the recording for up to 90 minutes.<br />4. Remove the mouse from the arena and clean the arena.<br />5. Score the behaviour of the animals for nocifensive behaviors between 10 and 60 minutes post formalin administration.","Purpose":"To assess nocifensive behaviours triggered by the inflammatory response following a formalin injection.","Experimental Design":"Minimum number of mutant animals: 5 males + 5 females<br />Age at test: 16 weeks","Notes":"This procedure can be performed as a terminal or non-terminal procedure. If terminal, euthanize the mouse humanely after the recording.<br /><br />This procedure is a pilot study from the Pain Phenotyping Pilot"}