{"Equipment":"<ol>\n\t<li>\n\t\tClinical chemistry analyser</li>\n\t<li>\n\t\tRefrigerated centrifuge</li>\n\t<li>\n\t\tEppendorf tubes</li>\n\t<li>\n\t\tPipettes (200-1000 ul)</li>\n</ol>\n","Procedure":"<p>\n\tSet up the clinical chemistry analyser and perform QC analyses of the control reagents in accordance with the equipment guidelines.</p>\n<p>\n\tSample collection and preparation:</p>\n<ol style=\"list-style-type:lower-alpha;\">\n\t<li>\n\t\tCollect the appropriate volume of blood required (160-200&mu;l of plasma), for the clinical chemistry analyser being used for assessment, in a&nbsp; BD Microtainer tube containing Lithium Heparin with the relevant blood collection procedure (see IMPC protocol Blood collection by retro-orbital puncture). Time of day for collection is in the morning, starting no earlier than 07:30.</li>\n\t<li>\n\t\tKeep whole blood samples on ice until centrifugation. If plasma samples cannot be analysed immediately, keep them in the fridge until analysis.</li>\n\t<li>\n\t\tAnalysis of samples is optimally done on the day of collection. When not possible the plasma samples can be stored at 2-8&deg;C. If samples require storage for &gt; 48 hours, freeze plasma at -20 &deg;C in single aliquots. All samples are allowed to come to room temperature prior to analysis.</li>\n\t<li>\n\t\tUse plasma samples undiluted or diluted to a ratio of 1:2 with deionised water if the volume is insufficient.</li>\n\t<li>\n\t\tbriefly centrifuge them at ~5000 x g for&nbsp;10 minutes. If necessary, remove fibrin clots using a wooden applicator.</li>\n</ol>\n","Purpose":"<p>\n\tClinical chemistry determines biochemical parameters in plasma including enzymatic activity, specific substrates and electrolytes.</p>\n<p>\n\tOntological description: MP:0001545 &ndash; blood physiology abnormalities.</p>\n<p>\n\t&nbsp;</p>\n","Experimental Design":"<div class=\"expandable\">\n\t<ul>\n\t\t<li>\n\t\t\t<strong>Minimum number of animals :</strong>&nbsp; 7M + 7F</li>\n\t\t<li>\n\t\t\t<strong>Age at test: </strong>Week 13 </li>\n\t\t<li>\n\t\t\t<strong>Sex:</strong> We would expect the results of this test to show sexual dimorphism</li>\n\t</ul>\n</div>\n<p>\n\t&nbsp;</p>\n","Notes":"<p>\n\tBlood collection for Clinical Chemistry and Hematology is performed as a fasting (after 4 hours) procedure, with some mice being used for subsequent gross pathology and other clinic-specific parameters included in terminal assessments.</p>\n<p>\n\tDilution. Dilution of blood is highly discouraged, but is allowed when the total necessary amount is not obtained. If dilution is necessary then the assays should be done in one run.</p>\n<ol>\n\t<li>\n\t\tPlasma samples must be free of Fibrin clots in order to be analysed.</li>\n\t<li>\n\t\tEach morning, all parameters are tested with control sera (see ESLIM_015_001_Annex_3: Controls for biochemistry on AU400). Some parameters are tested with control serum level 1 (Beckman Coulter System Reagent, ODC0003) and control serum level 2 (Beckman Coulter System Reagent, ODC0004), which consists of lyophilised human plasma with a normal and a pathological concentration. Other parameters are tested with specific controls from other suppliers.</li>\n\t<li>\n\t\tControls are thawed and vortexed before utilisation and loaded according to the analyser&rsquo;s display. Control values must lie within the acceptable range indicated by the manufacturer, otherwise the specific tests must be recalibrated and specific measurements repeated. Controls can be stored in 200&mu;l aliquots at -20&deg;C for up to 1 week.</li>\n</ol>\n"}