{"Procedure":"
    \n\t
  1. \n\t\tTransfer mice from animal room 4435 to the testing room 4435C
    2. \n\t
  2. \n\t\tMice are weighed, and 1 drop of Atropine is placed in right eye (unless eye is missing, small, or otherwise effected) to induce mydriasis.
    3. \n\t
  3. \n\t\tPlace mice in dark adaption chamber.\n\t\t
      \n\t\t\t
    1. \n\t\t\t\tAll rod testing procedures are done under dim red light after 2 hour dark adaptation
      2. \n\t\t
    \n\t
    4. \n\t
  4. \n\t\tKetaset/Xylazine /saline solution (0.1ml/20g body weight) is injected (i.p.) to induce anesthesia and 1 drop of Cyclomydril (ophthalmic solutions) are applied to induce mydriasis.
    5. \n\t
  5. \n\t\tOnce the pupil has dilated and anesthetic agents have taken affect, the mouse is placed on a heating pad (38°C) and the electrodes are attached\n\t\t
      \n\t\t\t
    1. \n\t\t\t\tTail (ground)
      2. \n\t\t\t
    2. \n\t\t\t\tLeft cheek (reference)
      3. \n\t\t\t
    3. \n\t\t\t\tCornea (recording)\n\t\t\t\t
        \n\t\t\t\t\t
      1. \n\t\t\t\t\t\tUse one drop of Goniosol to moisten cornea
        2. \n\t\t\t\t
      \n\t\t\t
      4. \n\t\t
    \n\t
    6. \n\t
  6. \n\t\tThe rod test is performed with a short-wavelength Wratten filter (# 47A), using stimulation intensities of 8 lumen sec/ft2, and 0.2 HZ frequency, every 10 seconds.
    7. \n\t
  7. \n\t\tData is saved and graphed
    8. \n\t
  8. \n\t\tAfter the dark-adapted tests are run, inject next mouse and put back into dark box.  Turn on a steady light to isolate cone responses in rod tested mouse.
    9. \n\t
  9. \n\t\tAfter 8-10 minutes of light adaptation, cone ERGs are obtained to white strobe flashes presented at 1 Hz, with intensities of 16 lumen sec/ft2, every 1 second for 20 seconds.
    10. \n\t
  10. \n\t\tCarefully remove the recording electrodes.  Anaesthesia reversal is promoted with a subQ injection of (~0.5ml) sterile saline and mouse recovers on a heating pad until fully conscious.
    11. \n\t
  11. \n\t\tData is saved and graphed
    12. \n\t
  12. \n\t\tOpen indexing program and index and save rods and cones tests.
    13. \n
\n","Purpose":"

\n\tFull-field electroretinogram (ERG) is a mass electrical response of the retina to a light stimulus.  The ERG contains two components, reflecting the response of the photoreceptors (a-wave) and ON bipolar cells (b-wave; Kofujj et al., 2000 J Neurosci 20: 5733-5740, 2000)

\n","Experimental Design":"
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