{"Equipment":"

\n\tColumbus Instruments Tail-Flick Analgesia Meter
\n\t70% Alcohol
\n\tClidox 1:5:1
\n\tSterile Cloths
\n\tWashcloths

\n","Procedure":"
    \n\t
  1. \n\t\tAnimals are transported to the anteroom and left undisturbed for 30 minutes before testing.
    2. \n\t
  2. \n\t\tThe apparatus is set to the auto-detect mode ensuring an automated tail flick detection when it occurs.
    3. \n\t
  3. \n\t\tThe mouse is then placed on the testing plate and covered with a sterile cloth while the tail remains exposed.
    4. \n\t
  4. \n\t\tThe tail is gently positioned into the groove. The green light beside the groove comes on indicating the correct placement of the tail.
    5. \n\t
  5. \n\t\tThe start button is then pressed initiating the timer. A high intensity beam of light is directed at mouse’s tail through a small hole in the groove.
    6. \n\t
  6. \n\t\tThe tail flick is detected automatically and the timer is stopped.
    7. \n\t
  7. \n\t\tThree different trials are run with inter-trial intervals lasting at least 60 seconds.
    8. \n\t
  8. \n\t\tThree trials are averaged to improve the accuracy of the test.
    9. \n
\n","Purpose":"

\n\tThe Tail Flick test is used to assess pain sensitivity (nociception). The time it takes for an animal to rapidly
\n\tremove the tail from an intense heat source is used as an index of peripheral pain response.

\n","Experimental Design":"
\n\t\n
\n

\n\t 

\n","Notes":"

\n\tThe maximum trial duration is set to 18 seconds to prevent any potential tissue damage.

\n

\n\tThe equipment is cleaned with Clidox before introducing a mouse from a different cage. Ethanol is used to remove any olfactory cues between testing of males and females. Clidox is then used to wipe off any remaining ethanol before the actual testing.

\n"}