\n\tMice were fed on Mouse Breeder Diet (5021,Labdiet) from weaning
\n\n\tAnalysis is performed on the buffy coat obtained from heparinised blood after red blood cell lysis for the following parameters: Percentages of total T cells (CD3+), CD4+ and CD8+ T cells, NKT cells (CD3+ CD161+), NK cells (CD3-CD161+), B cells (CD19+), Granulocytes (CD11b+ Gr1+) and Monocytes (CD11b+ Gr1dim/neg CD161-) are presented relative to the total viable CD45+ population.
\n\n\tPercentages of CD44hi CD62Llo CD4+ T cells and CD25+ regulatory T cells are presented relative to the total CD4+ T cell population.
\n\n\tPercentages of effector of CD44hi CD62Llo CD8+ T cells and mature IgD+ B cells are presented relative to the total CD8+ T cell and B cell populations respectively.
\n\n\tAll samples are acquired on a BD LSR II with dead cells excluded with the use of a viability indicator.
\n\n\tAll analysis is performed using FlowJo software.
\n","Purpose":"\n\tBuffy coat peripheral blood leukocyte immunophenotyping
\n"}