\n\t1. Clinical chemistry analyzer
\n\n\t2. 1.5 mL Eppendorf tubes
\n\n\t3. Pipettes (200-1000 ul)
\n","Data QC":"\n\tThese are the kits for calibrators and QC that Jax uses:
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\n| \n\t\t\t\t \n\t\t\t\t\tTest \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tAu # \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tTest kit # \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tCalibrator Kit # \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tQC Kit # \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tFrequency of Assay \n\t\t\t | \n\t\t
| \n\t\t\t\t \n\t\t\t\t\tCreatinine \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\t12. CRE \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tBeckman OSR6178 \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tBeckman DR0091 \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tBioRad 395X (2 levels) \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tCalibrator and Controls assayed for every day of a test run. \n\t\t\t | \n\t\t
| \n\t\t\t\t \n\t\t\t\t\tMicroAlbumin \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\t27. MALB \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tBeckman OSR6167 \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tBeckman ODR3024 \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tBioRad 395X (2 levels) \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tCalibrator and Controls assayed for every day of a test run. \n\t\t\t | \n\t\t
| \n\t\t\t\t \n\t\t\t\t\tMagnesium \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\t20.MG \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tBeckman OSR6789 \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tBeckman DR0090 \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tBioRad 395X (2 levels) \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tCalibrator and Controls assayed for every day of a test run. \n\t\t\t | \n\t\t
| \n\t\t\t\t \n\t\t\t\t\tGlucose \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\t92.GLUX \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tBeckman OSR6121 \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tBeckman DR0090 \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tBioRad 395X (2 levels) \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tCalibrator and Controls assayed for every day of a test run. \n\t\t\t | \n\t\t
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\n\n\tTo create a standard curve for calculating microalbumin:
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\n\n\tSigma Mouse Albumin (A3139-10MG) Dilution Procedure to create Standard Curve.
\n\n\t
\n\n\t1) Reconstitute with 1 mL Reagent Grade H2O
\n\n\t2) This Stock will = 1,000 mg/dL Microalbumin
\n\n\t3) Make a 2 X dilution of stock (resulting in new stock solution #2 of 500 mg/dL)
\n\n\t with Beckman Diluent 1 (PN# 442825)
\n\n\t4) Make a 10X dilution stock # 2 ( to new stock #3 of 50 mg/dL)
\n\n\t5) Perform serial dilution 1:1 of stock #3 to obtain the following series of standards for MicroAlbumin:
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\n\n\t6) Run standard samples on the AU680
\n\n\tUsing the MicroAlbumin Regression template, insert the measured values for the standards and redraw the regression line. Update the value in the divisor box to reflect the value in front of x from the regression line. The formula is set to divide each measured sample value by the divisor to calculate the known concentration (mg/dL). Then input the creatinine concentration (mg/dL) and it will calculate and convert the ratio (mg alb/g creatinine).
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\n","Procedure":"\n\tSet up the clinical chemistry analyzer and perform QC analyses of the control reagents in accordance with the equipment guidelines.
\n\n\tSample collection and preparation:
\n\n\ta. Collect the appropriate volume of urine required (70ul), for the clinical chemistry analyser being used for assessment, in a microcentrifuge tube Time of day for collection is in the morning, starting no earlier than 07:30. If required volume is not obtained the first day, repeat collection on the next day and combine with Day 1 sample for same mouse.
\n\n\tb. Keep whole urine samples on ice until analysis. If samples cannot be analysed immediately, keep them at 4°C until analysis.
\n\n\tc. Analysis of samples is optimally done on the day of collection. When not possible the samples can be stored at 2-8°C. If samples require storage for > 48 hours, freeze at -20 °C in single aliquots. All samples are allowed to come to room temperature prior to analysis.
\n\n\td. Use samples undiluted or diluted to a ratio of 1:1 with deionised water if the volume is insufficient.
\n","Purpose":"\n\tUrinalysis is performed to monitor renal function and will be performed as part of the adult phenotyping pipeline for KOMP2 mice. Samples will be analyzed using the Beckman AU680 autoanalyzer. Levels of urinary albumin, creatinine, magnesium and glucose are measured. The albumin to creatinine ratio (ACR) will be derived. A standard curve is run for microalbumin with each set of samples and used to derive an adjusted albumin value for use in the ACR.
\n","Experimental Design":"\n\n\t
\n","Notes":"\n\tUrine collection is performed as a non-fasting procedure, always in the morning.
\n\n\tDilution. Dilution of urine is highly discouraged, but is allowed when the total necessary amount is not obtained. If dilution is necessary then the assays should be done in one run.
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\n\n\tMetadata & examples
\n\n\t
\n| \n\t\t\t\t \n\t\t\t\t\tMetadata \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tExample \n\t\t\t | \n\t\t
| \n\t\t\t\t \n\t\t\t\t\tEquipment Name \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tID of the machine used when more than 1 is used having same model and manufacturer. E.g. machine 1, machine 2, machine Minnie, machine Mickey Mouse, etc. \n\t\t\t | \n\t\t
| \n\t\t\t\t \n\t\t\t\t\tEquipment manufacturer \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tManufacturer of the equipment. E.g. Olympus Diagnostics. \n\t\t\t | \n\t\t
| \n\t\t\t\t \n\t\t\t\t\tEquipment model \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tModel of the equipment. E.g. AU400, AU680 \n\t\t\t | \n\t\t
| \n\t\t\t\t \n\t\t\t\t\tAnaesthesia used for urine collection \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tThe drug used for anaesthesia during urine collection. E. g. Isofluorane. Or None. \n\t\t\t | \n\t\t
| \n\t\t\t\t \n\t\t\t\t\tMethod of urine collection \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tConcise description of the method used for urine collection. \n\t\t\t | \n\t\t
| \n\t\t\t\t \n\t\t\t\t\tSamples kept on ice between collection and analysis \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tYes/No. \n\t\t\t | \n\t\t
| \n\t\t\t\t \n\t\t\t\t\tSample status \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tIndicate if the sample were frozen (analysis on the same day of collection not possible) or fresh (analysis on the same day of collection). E.g Fresh/Frozen. \n\t\t\t | \n\t\t
| \n\t\t\t\t \n\t\t\t\t\tUrine dilution \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tDilution is highly discouraged but if necessary indicate the extent. E.g. No/1:1. \n\t\t\t | \n\t\t
| \n\t\t\t\t \n\t\t\t\t\tID of urine collection SOP \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tID of the protocol followed for urine collection. Can be a center specific protocol. E.g. ESLIM_024_001. \n\t\t\t | \n\t\t
| \n\t\t\t\t \n\t\t\t\t\tDate and time of urine collection \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tTime of day for collection is in the morning, starting no earlier than 07:30. E.g. Year, month, day, time. \n\t\t\t | \n\t\t
| \n\t\t\t\t \n\t\t\t\t\tDate of measurement \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tThe day of urine analysis. Year, month, day. \n\t\t\t | \n\t\t
| \n\t\t\t\t \n\t\t\t\t\tExperimenter ID \n\t\t\t | \n\t\t\t\n\t\t\t\t \n\t\t\t\t\tAn ID of any format to be used coherently both inside the same procedure and for all procedures. E.g. Harw_001, or 1/2/3. \n\t\t\t | \n\t\t
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