{"Procedure":"
\n\t1. Animals are transported to the testing room (or ideally an antechamber) and left undisturbed for 30 minutes before the test.
\n\t2. Ensure that lighting conditions are as desired (150-200 lux) and that all equipment is working correctly:
\n\t3. Testing is conducted during the light phase of the cycle with 1 hour gap from the light/dark change. Testing should be conducted during the same period of day.
\n\t4. Wipe the apparatus clean and allow time for it to dry
\n\t5. Each mouse is placed in the middle of a peripheral zone of the arena facing the wall and allowed to explore freely the apparatus, with the experimenter out of the animal's sight.
\n\tIf more than one mouse can be tested in parallel, in adjacent open field arenas and mice are video-tracked, it is important to ensure that the tracking of each mouse starts as soon as the mouse is released to make data comparable.
\n\n\tMales and females must be run in separate tests; ideally males are tested first, then followed by females
\n\n\t6. At the end of the 20 min run, animals are labeled (if necessary) and put back into their home cage.
\n\t7. After each run, any feces are removed and the arena is thoroughly wiped.
\n\t8. Analysis of the recording is done to measure the activity of each mouse in each of the zones per 5 minute bins.
\tThe Open Field test is used to assess anxiety and exploratory behaviors. It is based on the natural tendency of an animal to explore and to protect itself using avoidance which translates to a normal animal spending more time in the periphery of the Open Field arena than in the center (the most anxiogenic area).","Experimental Design":"
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\n","Notes":"\n\tData QC
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