{"Equipment":"<ol>\n\t<li>\n\t\tScale/balance</li>\n\t<li>\n\t\tFlow chambers</li>\n\t<li>\n\t\tPlethysmographs</li>\n\t<li>\n\t\tNebulizers</li>\n\t<li>\n\t\tComputer connected to flow chamber</li>\n\t<li>\n\t\tChallenge reagents</li>\n\t<li>\n\t\tPersonal safety equipment (masks, gloves, etc.)</li>\n</ol>\n","Procedure":"<ol>\n\t<li>\n\t\tBefore starting, make sure the chambers are calibrated</li>\n\t<li>\n\t\tWeigh&nbsp;the mouse and transfer to test room</li>\n\t<li>\n\t\tTurn on amplifier, nebulizer and computer</li>\n\t<li>\n\t\tPlace the mouse in the chamber</li>\n\t<li>\n\t\tMethacholine challenge:\n\t\t<ol>\n\t\t\t<li>\n\t\t\t\tNebulize with PBS for 2 minutes</li>\n\t\t\t<li>\n\t\t\t\tMeasure response to PBS for 5 minutes</li>\n\t\t\t<li>\n\t\t\t\tNebulize with 12.5mg/ml MCh for 2 minutes</li>\n\t\t\t<li>\n\t\t\t\tMeasure response to 12.5mg/ml for 5 minutes</li>\n\t\t\t<li>\n\t\t\t\tNebulize with 25mg/ml MCh for 2 minutes</li>\n\t\t\t<li>\n\t\t\t\tMeasure response to 25mg/ml for 5 minutes</li>\n\t\t\t<li>\n\t\t\t\tNebulize with 50mg/ml MCh for 2 minutes</li>\n\t\t\t<li>\n\t\t\t\tMeasure response to 50mg/ml MCh for 5 minutes</li>\n\t\t</ol>\n\t</li>\n\t<li>\n\t\tAdditional optional&nbsp;hypoxia challenge:\n\t\t<ol>\n\t\t\t<li>\n\t\t\t\tMeasure response unchallenged for 5 minutes</li>\n\t\t\t<li>\n\t\t\t\tMeasure response baseline for 5 minutes</li>\n\t\t\t<li>\n\t\t\t\tMeasure response to challenge with 10% O2 for 5 minutes</li>\n\t\t\t<li>\n\t\t\t\tMeasure response to challenge with 21% O2 for 5 minutes</li>\n\t\t</ol>\n\t</li>\n\t<li>\n\t\tRemove the mouse from its chamber and place back in the home cage.</li>\n</ol>\n","Purpose":"<p>\n\tThe purpose of this procedure is to record the respiratory function of mice, when submitted to methacholine or hypoxia challenge. Other similar protocols, for allergen sensitization and for LPS challenges, will also be available.</p>\n<div>\n\t&nbsp;</div>\n<div>\n\tOntological description: &nbsp;MP:0002327 - abnormal respiratory function</div>\n","Experimental Design":"<div class=\"expandable\">\n\t<ul>\n\t\t<li>\n\t\t\t<strong>Minimum number of animals :</strong>&nbsp; 4M + 4F</li>\n\t\t<li>\n\t\t\t<strong>Age at test: </strong>Week 13 </li></ul>\n</div>\n<p>\n\t&nbsp;</p>\n","Notes":"<p>\n\tIf any animal is manifesting difficulties at any stage of the procedure, regardless of the test, it should be removed from the nebulizing chamber and allowed to recover. It should not go through the rest of the test nor re-tested.</p>\n<p>\n\tAerosolized methacholine leads to bronchoconstriction; Asthmatic patients should not handle this substance nor be present in the room where the challenge takes place due to high risk of asthma attack and health hazard.</p>\n"}