{"Equipment":"<ul>\n\t<li>\n\t\tfine forceps</li>\n\t<li>\n\t\tsurgical scissors</li>\n\t<li>\n\t\tfine surgical scissor</li>\n\t<li>\n\t\tkim wipes (tissues) or surgical compress</li>\n\t<li>\n\t\tlaboratory balance</li>\n\t<li>\n\t\tlabelled jar with fixative</li>\n\t<li>\n\t\tcorkplate or wax board</li>\n\t<li>\n\t\tpins</li>\n\t<li>\n\t\tjar containing tap water to rinse the tools</li>\n</ul>\n","Procedure":"<p>Methods and procedures used not including center-specific data entry methods.</p><p>1. Sacrifice the mouse</p><p>2. Weigh and record total body weight</p><p>3. Place mouse on its back and pin the mouse onto board with extended extremities (inner side of hands and foot)</p><p>4. Wipe or wet the mouse with 70% ethanol to control hair and dander</p><p>5. Option A (for mice that are undergoing complete necropsy):</p><ul><li>Proceed with a complete necropsy and tissue collection according to Centre-specific technical SOP including removal of the heart by dissecting the aortic root immediately above the aortic valves and the superior vena cava above the atria</li><li>Remove adjacent mediastinal fat pads from the excised heart carefully with forceps</li><li>Empty heart blood by tapping the heart on a kim wipe (absorbent pad) or surgical compress. Repeat until the heart is totally dry</li><li>Weigh the heart, record the weight in the Centre-specific database, and place the heart in fixative</li></ul><p>6. Option B (for mice that are not undergoing complete necropsy):</p><ul><li>Open the skin in the ventral midline and in the direction of the extremities and extend cut to hands and feet</li><li>Open the muscular abdominal wall in the midline and along the lower margin of the rib cage with small forceps</li><li>Open rib cage by removing the sternum and adjacent ribs</li><li>Remove the heart by dissecting the aortic root immediately above the aortic valves and the superior vena cava above the atria</li><li>Remove adjacent mediastinal fat pads from the excised heart carefully with forceps</li><li>Empty heart blood by tapping the heart on a kim wipe (absorbent pad) or surgical compress. Repeat until the heart is totally dry</li><li>Weigh the heart and record the weight in the Centre-specific database</li><li>Discard the heart</li></ul><p><img src=\"https://api.mousephenotype.org/impress/image/e95f4c9ba3d5379b8d3808d083860c505cef3a33ffa321f29e01ba8f535b7882\"></p>","Purpose":"<p>\r\n\tTo evaluate cardiac size using heart weight and body weight.</p>\r\n","Experimental Design":"<div class=\"expandable\">\n\t<ul>\n\t\t<li>\n\t\t\t<strong>Minimum number of animals :</strong>&nbsp; 7M + 7F</li>\n\t\t<li>\n\t\t\t<strong>Age at test: </strong>Week 16 </li></ul>\n</div>\n<p>\n\t&nbsp;</p>\n","Notes":"<p>\n\tAll data are collected at a local workstation in the necropsy room (attached to a digital balance) and uploaded to the Centre-specific pathology data capture system.</p>\n<p>\n\t<strong>Data QC</strong><br />\n\t<br />\n\tMouse weight between 5 grams and 150 grams</p>\n<p>\n\t<strong>IMPC Parameters (+ontology annotations)</strong><br />\n\t<br />\n\tWeight (in mg)</p>\n<p>\n\t<strong>Data Analysis, annotation and display (+statistics)</strong></p>\n<p>\n\tStatistics: ANOVA/Wilcoxon test using normalized heart weights (for body weight) displayed in boxplot</p>\n"}