{"Procedure":"<p>\r\n\tCenter-specific&nbsp; methods will be provided and be on file and linked to the data for each mutant line.</p>\r\n<p>\r\n\tFor each mouse, for each tissue, the scoring system will be: &nbsp;+ (specific staining), - (no staining), NS (non-specific staining), or NA (tissue not available)</p>\r\n<p>\r\n\t<em>Where positive, specific, staining is found:</em></p>\r\n<p>\r\n\tA.&nbsp; A representative image will be taken and uploaded for each mutant line.&nbsp; The image will have a sizing reference bar and optional center-specific watermark embedded into the image.</p>\r\n<p>\r\n\tB. The metadata collected for each mouse will be associated with the image.</p>\r\n<p>\r\n\tC. Each image will have a substructure field for additional annotation provided by that center.</p>\r\n","Purpose":"<p>\r\n\t<strong>To stain adult mutant&nbsp; and control tissues for bacterial beta-galactosidase (LacZ) activity in order to assess the adult organ, tissue, substructure and cell type of gene expression of IKMC alleles utilizing a LacZ reporter.&nbsp; </strong></p>\r\n<p>\r\n\t<strong>Description: </strong>Adult mouse tissues are scored for presence of LacZ staining which is distinct from either nonspecific staining observed in wildtype control mice, or is too faint to score as present.&nbsp; Intensity of staining is not required but can be reported.&nbsp;&nbsp; Anatomical terms to localize staining to the organ, structure, substructure, or cell type will use standard mouse anatomy ontology terms.</p>\r\n","Experimental Design":"<p>Mutants:</p>\r\n<ul>\r\n<li><strong>Minimum number of animals :</strong>&nbsp;1M or1F heterozygote</li>\r\n<li>Age will depend upon the logistics of each center, but all mice will be within 7 days of the age stipulated in the center-specific protocols, and all mice will be &gt; 50 days of age;</li>\r\n<li>The tissues in the agreed parameter list will be stained and annotated for each mutant</li>\r\n</ul>\r\n<p>Controls:</p>\r\n<ul>\r\n<li>A minimum of one control mouse will be stained and annotated for every 10 mutants</li>\r\n<li>The tissues in the agreed parameter list will be stained and annotated for each control</li>\r\n</ul>","Notes":"<p>\r\n\tTables of results for each tissue from each HET or HOM mouse scored for:&nbsp; presence/absence/non-specific/tissue not-available as defined above.</p>\r\n<p>\r\n\tRepresentative images for positive staining tissues.</p>\r\n<p>\r\n\tAdditional annotation to provide anatomical substructure &amp; cell specificity.</p>\r\n<p>\r\n\tDatabase of non-specific staining and presentation of images representative of non-specific staining.</p>\r\n<p>\r\n\t&nbsp;</p>\r\n<p>\r\n\t<strong>Data QC</strong></p>\r\n<p>\r\n\tQuality control for each center&rsquo;s processes and annotation will be established by that center&rsquo;s staff and published as part of their detailed protocol.</p>\r\n<p>\r\n\tA database of control non-specific staining will be maintained by the Data Coordination Center and updated periodically to indicate the frequency of non-specific staining found in wildtype mice by each center.&nbsp; Representative images of non-specific staining will be linked to the data for each mutant line.</p>\r\n"}