{"Procedure":"

\t1.    Monitor pup number, genotypes and sex ratios of Het X Het intercrosses set to generate cohorts for phenotyping. Score at least 28 live pups when genotyped, unless four or more hom pups are produced before this threshold is reached.
\t2.    Identify strains that produce no homozygous/hemizygous male or female pups.
\t    a.    Strains that produce NO homozygous pups will be considered LETHAL (complete preweaning lethality [MP: 0011100]).
\t    b.    X-linked strains that produce NO hemizygous male pups and NO female homozygous pups will be considered LETHAL (complete preweaning lethality [MP: 0011100]).
\t    c.    These will undergo embryonic lethal pipeline (if available)
\t3.    Identify strains that produce less than normal numbers of homozygous/hemizygous male or female pups.
\t    a.    Strains that produce <50% expected (#totalpups * 0.125 (3 for 28) (4 for 29-36) (5 for 37-52) (See stats table in Notes)) homozygous pups will be considered SUBVIABLE (partial preweaning lethality [MP: 0011110]).
\t    b.    X-linked strains that produce <50% expected (#total pups* 0.125 (3 for 28) (4 for 29-36) (5 for 37-52) (See stats table in Notes)) hemizygous male pups and  female homozygous pups will be considered SUBVIABLE (partial preweaning lethality [MP: 0011110]).
\t    c.    Some centers will proceed with secondary screening.
\t4.    For lethal and subviable strains, heterozygous progeny will be sent for adult phenotyping.
\t 

","Purpose":"

\tTo assess the postnatal viability, sub-viability, and lethality of homozygous mice during cohort production.","Experimental Design":"

","Notes":"

\tAll genotypes should be collected using validated assays.
\tLine level calls will be rejected until 28 mice have been genotyped, unless four or more hom pups are produced before this threshold is reached, in which case a viable call is valid.
\t 

\tSub-viable significance table:

\t 

\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t
Number genotyped\t\t\tPups observed\t\t\tFormula (Excel)\t\t\tP-value\t\t\t
28\t\t\t3\t\t\t=BINOMDIST(3,28,0.25,1)\t\t\t0.055135567\t\t\t
29\t\t\t4\t\t\t=BINOMDIST(4,29,0.25,1)\t\t\t0.115324345\t\t\t
\t\t\t\t\t\t\t\t\t30\t\t\t\t\t\t\t\t\t\t\t\t4\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(4,30,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.0978696\t\t\t
\t\t\t\t\t\t\t\t\t31\t\t\t\t\t\t\t\t\t\t\t\t4\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(4,31,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.082764531\t\t\t
\t\t\t\t\t\t\t\t\t32\t\t\t\t\t\t\t\t\t\t\t\t4\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(4,32,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.069757389\t\t\t
\t\t\t\t\t\t\t\t\t33\t\t\t\t\t\t\t\t\t\t\t\t4\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(4,33,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.05860841\t\t\t
\t\t\t\t\t\t\t\t\t34\t\t\t\t\t\t\t\t\t\t\t\t4\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(4,34,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.049093333\t\t\t
\t\t\t\t\t\t\t\t\t35\t\t\t\t\t\t\t\t\t\t\t\t4\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(4,35,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.041005517\t\t\t
\t\t\t\t\t\t\t\t\t36\t\t\t\t\t\t\t\t\t\t\t\t4\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(4,36,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.034156964\t\t\t
\t\t\t\t\t\t\t\t\t37\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(5,37,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.071139152\t\t\t
\t\t\t\t\t\t\t\t\t38\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(5,38,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.060448988\t\t\t
\t\t\t\t\t\t\t\t\t39\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(5,39,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.051216574\t\t\t
\t\t\t\t\t\t\t\t\t40\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(5,40,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.043273983\t\t\t
\t\t\t\t\t\t\t\t\t41\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(5,41,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.036466047\t\t\t
\t\t\t\t\t\t\t\t\t42\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(5,42,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.030650935\t\t\t
\t\t\t\t\t\t\t\t\t43\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(5,43,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.025700232\t\t\t
\t\t\t\t\t\t\t\t\t44\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(5,44,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.021498648\t\t\t
\t\t\t\t\t\t\t\t\t45\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(5,45,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.017943462\t\t\t
\t\t\t\t\t\t\t\t\t46\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(5,46,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.014943774\t\t\t
\t\t\t\t\t\t\t\t\t47\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(5,47,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.012419646\t\t\t
\t\t\t\t\t\t\t\t\t48\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(5,48,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.010301181\t\t\t
\t\t\t\t\t\t\t\t\t49\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(5,49,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.008527583\t\t\t
\t\t\t\t\t\t\t\t\t50\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(5,50,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.007046225\t\t\t
\t\t\t\t\t\t\t\t\t51\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(5,51,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.005811761\t\t\t
\t\t\t\t\t\t\t\t\t52\t\t\t\t\t\t\t\t\t\t\t\t5\t\t\t\t\t\t\t\t\t\t\t\t=BINOMDIST(5,52,0.25,1)\t\t\t\t\t\t\t\t\t\t\t\t0.004785276\t\t\t

\t 

"}