{"Procedure":"<ol>\n\t<li>\n\t\tEmbryos are stained in whole-mount according to centre-specific protocols. Centre-specific methods will be provided and be on file and linked to the data for each mutant line</li>\n\t<li>\n\t\tIf there is no staining, all embryos are genotype confirmed.</li>\n\t<li>\n\t\tWhen positive staining is found, a representative image will be taken and uploaded for each mutant line.&nbsp; If heterozygous and homozygous embryos are stained, a representative image of each can be uploaded. The image will have a sizing reference bar.</li>\n\t<li>\n\t\tThe metadata collected for embryonic litter will be associated with the image</li>\n\t<li>\n\t\tAnnotations will derive from the heterozygous embryo only.</li>\n\t<li>\n\t\tFor each embryo the scoring system will be: &nbsp;&ldquo;Expression&rdquo; (specific staining), &ldquo;No Expression&rdquo;&nbsp; (no staining), &ldquo;Ambiguous&rdquo; (non-specific, not clear), &ldquo;imageOnly&rdquo;. or&nbsp; &quot;<span class=\"multi\">tissue not available&quot;</span></li>\n</ol>\n<p>\n\tIf capturing images please attempt to capture left, right, front, and back views of the embryo.</p>\n","Purpose":"<p>\n\t<strong>To stain mutant and control embryos for bacterial beta-galactosidase (LacZ) activity in order to assess the embryonic gene expression of IKMC alleles at embryonic day 12.5 (E12.5).&nbsp; </strong></p>\n<p>\n\t<strong>Description:</strong> Whole-mount or sections of stained E12.5 embryos are scored for presence of LacZ staining. Intensity of staining is not required but can be reported. Anatomical terms to localize staining to the structure or substructure will use standard EMAP ontology terms.</p>\n","Experimental Design":"<p>\n\tOutcrosses (Het x WT) or intercrosses (Het x Het) may be used for the protocol.</p>\n<p style=\"margin-left:36.0pt;\">\n\tFor outcrosses, genotype confirmation should be performed if no embryos display positive staining.</p>\n<p style=\"margin-left:36.0pt;\">\n\tFor intercrosses, genotype confirmation must be performed on ALL samples to distinguish heterozygous and homozygous stained embryos.</p>\n<p>\n\t&nbsp;</p>\n<p>\n\tMutants:</p>\n<ul>\n\t<li>\n\t\tEmbryos are harvested, fixed, processed, and stained according to centre-specific protocols</li>\n\t<li>\n\t\tEmbryos with staining are scored and imaged, concurrent with genotyping to determine zygosity and to identify controls</li>\n\t<li>\n\t\t\t<strong>Minimum number of animals :</strong>&nbsp; 1 mutant of any sex</li>\n\t\t<li>\n\t\t\t<strong>Age at test: </strong>E12.5</li>\n</ul>\n<p>\n\tControls:</p>\n<ul>\n\t<li>\n\t\tWild type littermate E12.5 embryos that do not exhibit any endogenous mammalian b-galactosidase activity are used as controls. C57BL/6N litters can be stained at each centre&rsquo;s discretion.</li>\n</ul>\n","Notes":"<h3>\n\tData QC</h3>\n<p>\n\tQuality control for each centre&rsquo;s processes and annotation will be established by that centre&rsquo;s staff and published as part of their detailed protocol</p>\n"}