{"Procedure":"
\n\tIf capturing images please attempt to capture left, right, front, and back views of the embryo.
\n","Purpose":"\n\tTo stain mutant and control embryos for bacterial beta-galactosidase (LacZ) activity in order to assess the embryonic gene expression of IKMC alleles at embryonic day 12.5 (E12.5).
\n\n\tDescription: Whole-mount or sections of stained E12.5 embryos are scored for presence of LacZ staining. Intensity of staining is not required but can be reported. Anatomical terms to localize staining to the structure or substructure will use standard EMAP ontology terms.
\n","Experimental Design":"\n\tOutcrosses (Het x WT) or intercrosses (Het x Het) may be used for the protocol.
\n\n\tFor outcrosses, genotype confirmation should be performed if no embryos display positive staining.
\n\n\tFor intercrosses, genotype confirmation must be performed on ALL samples to distinguish heterozygous and homozygous stained embryos.
\n\n\t
\n\n\tMutants:
\n\n\tControls:
\n\n\tQuality control for each centre’s processes and annotation will be established by that centre’s staff and published as part of their detailed protocol
\n"}