{"Procedure":"
\n\tTo assess the viability, sub-viability, and lethality of homozygous embryos at E12.5
\n","Experimental Design":"\n\tAll genotypes should be collected using validated assays.
\n\n\tY chromosome assay required for X-linked lethal strains.
\n\n\t
\n\n\tPreliminary: No analysis required as it is a line level procedure. This could change with additional data about the procedure
\n\n\tSee E12.5 Gross Morphology protocol for MP calls of specific phenotypes at this time point.
\n\n\tTotal Embryos: All, WT, Het, Hom
\n\t •Alive, dead, and defect (all genotyped)
\n\tTotal Dead: All, WT, Het, Hom
\n\t
\n\tTotal Defect (Alive or Dead): All, WT, Het, Hom
\n\t •Abnormal and dead embryos
\n\tLitter size: all genotyped embryos
\n\t •ignore partials and reabsorptions.