{"Procedure":"<div class=\"expandable\">\n\t<ol>\n\t\t<li>\n\t\t\t<strong>Set up timed mating with heterozygous animals. Dissect at a consistent time and collect &gt;=2 placentas from homozygote embryos. Coordination with viability screen is at the centres discretion. </strong></li>\n\t\t<li>\n\t\t\t<strong>Assess placentas according to Gross Morphology parameters.</strong></li>\n\t\t<li>\n\t\t\t<strong>Generate gross images of placentas (optional) with scored defects and control placentas.</strong></li>\n\t\t<li>\n\t\t\t<strong>Collect tissue for genotyping </strong></li>\n\t\t<li>\n\t\t\t<strong>Process placentas for Histopathology, or other imaging (OPTIONAL - depending on center pipeline)</strong></li>\n\t\t<li>\n\t\t\t<strong>Scores will be shown per placenta and split by zygosity.</strong></li>\n\t</ol>\n</div>\n<p>\n\t&nbsp;</p>\n","Purpose":"<div class=\"expandable\">\n\t<p>\n\t\tTo assess visible morphological defects in E12.5 placentas from lethal strains</p>\n</div>\n<p>\n\t&nbsp;</p>\n","Experimental Design":"<div class=\"expandable\">\n\t<ul>\n\t\t<li>\n\t\t\tSet up timed matings with heterozygous mice</li>\n\t\t<li>\n\t\t\tDay 0 is defined as the midpoint of the prior dark cycle following the identification of a copulation plug.</li>\n\t\t<li>\n\t\t\t<strong>Minimum number of animals :</strong>&nbsp; 1 mutant of any sex</li>\n\t\t<li>\n\t\t\t<strong>Age at test: </strong>E12.5</li>\n\t\t<li>\n\t\t\tCapture gross images (<strong>optional</strong>)</li>\n\t\t<li>\n\t\t\tCollect tissue and genotype embryos.</li>\n\t</ul>\n</div>\n<p>\n\t&nbsp;</p>\n","Notes":"<div class=\"expandable\">\n\t<p>\n\t\tAll genotypes should be collected using validated assays.</p>\n\t<p>\n\t\tY chromosome assay required for X-linked lethal strains.</p>\n\t<p>\n\t\tPlacentas may be processed for Histopathology or 3D Imaging</p>\n</div>\n<p>\n\t&nbsp;</p>\n"}