{"Procedure":"<ol>\n\t<li>\n\t\t<strong>Set up timed mating with heterozygous animals. Dissect and collect &gt;=3 homozygote embryos. </strong></li>\n\t<li>\n\t\t<strong>Process the embryos using the centre&rsquo;s staining and embedding protocol . Centre-specific methods&nbsp; are recorded within a document</strong></li>\n\t<li>\n\t\t<strong>Scan and generate reconstructions of embryos.</strong></li>\n</ol>\n","Purpose":"<p>\n\tTo assess morphological defects in E14.5-E15.5 embryos from lethal strains with MicroCT.</p>\n","Experimental Design":"<ul>\n\t<li>\n\t\tSet up timed matings with heterozygous mice</li>\n\t<li>\n\t\tDay 0 is defined as the midpoint of the prior dark cycle following the identification of a copulation plug.</li>\n\t<li>\n\t\tCollect embryos at E14.5 or E15.5 with 3 minimum homozygous and 1 littermate control</li>\n\t<li>\n\t\tEnsure embryos are bleed as much as possible.</li>\n\t<li>\n\t\tReconstruct the scans according to manufacturer&#39;s instructions.</li>\n</ul>\n","Notes":"<p>\n\tEmbryos may be processed for Histopathology.</p>\n"}