{"Procedure":"
\n\t\tIf capturing images please attempt to capture left, right, front, and back views of the embryo but if this is not possible left, right is sufficient. Feel free to take higher magnification views to show morphologies of interest.
\n\n\t
\n","Purpose":"\n\t\tTo assess visible morphological defects in E9.5 embryos from lethal strains
\n\n\t
\n","Experimental Design":"\n\t
\n","Notes":"\n\t\tTam somite method for counting somites should be adopted:
\n\t\n\t\tTam scoring system uses a forelimb range of 8 to 15 somite pairs resulting in E9.5 embryos ranging between 25 to 26 somite pairs.
\n\t\n\t\t
\n\t\n\t\tAll genotypes should be collected using validated assays.
\n\t\n\t\tY chromosome assay required for X-linked lethal strains.
\n\t\n\t\tEmbryos may be processed for Histopathology or 3D Imaging
\n\n\t
\n"}