{"Data QC":"<p>\n\tLocal QC data to assure all data fields have recorded data.&nbsp; In the case all data fields have a value of 0 that particular mouse data will be removed.</p>\n<p>\n\t1.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Verify the total time of each run</p>\n","Procedure":"<p>\n\t1.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Light levels are set so that the light intensity within the light arena is between 100 &ndash; 200 lux and the dark arena is at 4-7 lux. Light metering is performed at the start of each test day.</p>\n<p>\n\t2.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; The method of recording is set up with each arena defined. The light arena is approximately one third to half of entire chamber.</p>\n<p>\n\t3.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; One mouse is placed in each <strong><em>light arena </em></strong>and recording is started.</p>\n<p>\n\t4.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; A minimum of 5 minute should be recorded up to a maximum of 20 minutes.</p>\n<p>\n\t5.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; At the end of the trial, the mice are removed from the arena and returned to their home cages.</p>\n<p>\n\t6.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; The chambers are cleaned with disinfectant between mice to decontaminate and remove scent cues from previously tested mice.</p>\n<p>\n\t7.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Analysis of the recording is done to measure the activity of each mouse in each of the two zones and the number of transitions between zones.&nbsp;</p>\n","Purpose":"<p>\tTo monitor the anxiety-related behaviour of mice in a two-chambered light-dark arena over a defined period of time in order to assess the anxiety phenotype.","Experimental Design":"<div class=\"expandable\">\n\t<ul>\n\t\t<li>\n\t\t\t<strong>Minimum number of animals :</strong>&nbsp; 7M + 7F</li>\n\t\t<li>\n\t\t\t<strong>Age at test: </strong>Week 10 </li>\n\t\t<li>\n\t\t\t<strong>Sex:</strong> We would expect the results of this test to show sexual dimorphism</li>\n\t</ul>\n</div>\n<p>\n\t&nbsp;</p>\n","Notes":"<p>\n\tLocal data processing may be implemented to calculate derived measures from this data.</p>\n"}